Description

Ultra-Active Nuclease (EC 3.1.30.2, commonly known as Benzonase) is a homodimer, with each subunit having a molecular weight of 24.8 kDa. It exhibits exceptional capability to degrade both DNA and RNA present in cell lysates and analytical samples while leaving proteins intact. Its high activity makes it highly versatile, leading to widespread applications in downstream purification workflows to reduce viscosity and achieve high yield and purity. These applications extend to the production of protein drugs, antibodies, vaccines, and viral vectors. Whether in laboratory research or industrial-scale production, Benzonase consistently delivers reliable performance across a wide range of conditions.

Key Features

1. Versatility Across Applications: Ultra-active nuclease efficiently cleaves all forms of nucleic acids (both DNA and RNA) into smaller oligonucleotides with 3-5 bases without sequence specificity, making it a versatile tool for applications ranging from research to large-scale biopharmaceutical processing.
2. High Activity and Fast-Acting: Ultra-active nuclease exhibits rapid efficiency in degrading nucleic acids without requiring extended incubation, thereby minimizing the risk of protease degradation.
3. Broad Compatibility in Harsh Conditions: Ultra-active nuclease is effective under a wide range of conditions, making it suitable for challenging environments and complex samples.
4. High Resistance to Inhibition: It remains resistant to DNase and RNase inhibitors, allowing it to function even when other nucleases become inactive. These characteristics make it one of the most widely applied enzymes in both biotechnological and pharmaceutical settings.

Sequence

Theoretical pI/Mw

6.23 / 26709.95