Affinity Map: A Robust Platform for Quantitative Protein-Ligand Binding Profiling
Protein-ligand binding, selectivity, and affinity govern the cellular effects of drugs and endogenous molecules. Current methods typically rely on qualitative omics analysis followed by affinity measurements using purified proteins or engineered reporters. Few approaches enable simultaneous target identification and quantitative affinity measurement, and those are often limited to specific enzyme classes or ligand-induced stability shifts. […]