Proteinase K (EC: 3.4.21.64) is a highly active serine protease with a molecular weight of approximately 28.9 kDa. It is well-known for its broad-spectrum ability to digest proteins, including various nucleases, during sample preparation, thereby preserving the integrity of nucleic acids. Proteinase K hydrolyzes peptide bonds adjacent to aliphatic, aromatic, or hydrophobic amino acids, generating smaller peptide fragments. It remains active even under denaturing conditions or elevated temperatures, making it ideal for isolating high-quality DNA and RNA from various biological sources. Due to its efficiency and reliability, Proteinase K is a versatile tool in molecular biology applications, ranging from gene cloning to genetic sequencing and diagnostic testing.
Key Features
Broad-Spectrum Proteolytic Activity: Efficiently digests a wide range of proteins, including nucleases, while preserving sample integrity during DNA and RNA extraction and genetic testing.
Wide Compatibility: Maintains robust enzymatic activity under a broad range of cleavage conditions, including varying pH levels, high temperatures, and denaturing environments—ideal for complex sample preparations.
Essential for Molecular Biology Applications: Effectively removes protein contaminants from various samples, enabling the preparation of high-quality nucleic acids and enhancing product quality, testing accuracy and sensitivity.
Proteinase K is available for research and laboratory use. To place an order or inquire about bulk options, please visit our website https://minnebio.com/product/protein-removal-enzyme/ for details.
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