Scientists have developed mScarlet3-H, a next-generation monomeric red fluorescent protein (RFP) that overcomes major limitations of existing RFPs. Engineered for exceptional stability, mScarlet3-H retains strong fluorescence under extreme conditions, including high temperatures, chaotropic stress, and oxidative environments. Its high-resolution structure (1.5 Å) has been resolved, enabling further structural insights.
Correlative Light and Electron Microscopy (CLEM): Robust fluorescence for multimodal imaging.
Fast Whole-Organ Clearing: Enables clearing in less than 24 hours.
Extended 3D SIM Imaging: Visualizes mitochondria with minimal photobleaching.
High-Quality Dual-Color STED Imaging: Strong signal with excellent specificity.
Benchmarking studies show mScarlet3-H outperforms leading RFPs in photostability and resilience, making it ideal for multimodal imaging across biological systems such as zebrafish, mice, and plants.
📖 Reference:
Yang, J. et al. Ultrastable monomeric red fluorescent protein enables multimodality imaging. Nat. Methods 21, 796–805 (2024).
https://doi.org/10.1038/s41592-024-02280-7
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